6SE6

Class2 : CENP-A nucleosome in complex with CENP-C central region


ChainDomainClassArchitectureTopologyHomology
A1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
E1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
B1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
F1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
C1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
G1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
D1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A
H1.10.20.10 Mainly Alpha Orthogonal Bundle Histone, subunit A Histone, subunit A

Protein Family Annotation Pfam Database Homepage

ChainsAccessionIdentifierDescriptionCommentsSource
A, EPF00125Core histone H2A/H2B/H3/H4 (Histone)Core histone H2A/H2B/H3/H4- Domain
B, FPF15511Centromere kinetochore component CENP-T histone fold (CENP-T_C)Centromere kinetochore component CENP-T histone foldCENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N-terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly asso ...CENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N-terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly associate with CENP-A, but with histone H3 in the centromere region. CENP-T and -W form a hetero-tetramer with CENP-S and -X and bind to a ~100 bp region of nucleosome-free DNA forming a nucleosome-like structure. The DNA-CENP-T-W-S-X complex is likely to be associated with histone H3-containing nucleosomes rather than with CENP-nucleosomes. This domain is the C-terminal histone fold domain of CENP-T, which associates with chromatin [2-3].
Domain
C, GPF16211C-terminus of histone H2A (Histone_H2A_C)C-terminus of histone H2A- Family
C, GPF00125Core histone H2A/H2B/H3/H4 (Histone)Core histone H2A/H2B/H3/H4- Domain
D, HPF00125Core histone H2A/H2B/H3/H4 (Histone)Core histone H2A/H2B/H3/H4- Domain
KPF15620Centromere assembly component CENP-C middle DNMT3B-binding region (CENP-C_mid)Centromere assembly component CENP-C middle DNMT3B-binding region- Family
KPF15622Kinetochore assembly subunit CENP-C N-terminal (CENP_C_N)Kinetochore assembly subunit CENP-C N-terminal- Family
KPF11699Mif2/CENP-C like (CENP-C_C)Mif2/CENP-C likeCENP-C_C is a C-terminal family of fungal and eukaryote proteins necessary for centromere formation. CENP-C is the inner-kinetochore centromere (CEN) binding protein. In the budding-yeast, Mif2, the yeast homologue, binds in the CDEIII region of the ...CENP-C_C is a C-terminal family of fungal and eukaryote proteins necessary for centromere formation. CENP-C is the inner-kinetochore centromere (CEN) binding protein. In the budding-yeast, Mif2, the yeast homologue, binds in the CDEIII region of the centromere, and has been shown to recruit a substantial subset of all inner and outer kinetochore proteins [1]. Mif2 adopts a cupin fold and is extremely similar both in polypeptide chain conformation and in dimer geometry to the dimerisation domain of a bacterial transcription factor [1]. The Mif2 dimer appears to be part of an enhanceosome-like structure that nucleates kinetochore assembly in budding yeast [1]. This C-terminal domain is the region via which CENP-C localises to centromeres throughout the cell cycle 2,3].
Domain

Gene Product Annotation Gene Product Annotation

ChainsPolymerMolecular FunctionBiological ProcessCellular Component
A, EHistone H3-like centromeric protein A
B, FHistone H4
C, GHistone H2A type 2-A
D, HHistone H2B type 1-C/E/F/G/I
IDNA (145-MER)none none none
JDNA (145-MER)none none none
VCentromere protein C