ELECTRON MICROSCOPY Experimental Data



EM Sample
Sample Vitrification Details --
Sample Aggregation State PARTICLE
Sample Reconstruction Method SINGLE PARTICLE
Name of Sample Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) Dodecamer in complex with Mg2+, NAPDH, and CPD at pH7.5
EM Data Acquisition
Date of Experiment --
Temperature (Kelvin) --
Microscope Model FEI TALOS ARCTICA
Detector Type FEI FALCON II (4k x 4k)
Minimum Defocus (nm) --
Maximum Defocus (nm) --
Minimum Tilt Angle (degrees) --
Maximum Tilt Angle (degrees) --
Nominal CS --
Imaging Mode BRIGHT FIELD
Electron Dose (electrons per Å**-2) 1.4
Illumination Mode FLOOD BEAM
Nominal Magnification --
Calibrated Magnification --
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details --
3D Reconstruction
Software Package(s) cisTEM
Reconstruction Method(s) --
EM Image Reconstruction Statistics
Number of Particles 35117
Other Details --
Effective Resolution 3.35
EM Map-Model Fitting and Refinement
Refinement Space Refinement Protocol Refinement Target Overall B Value Fitting Procedure Fitting Software
REAL RIGID BODY FIT -- 134.17 -- --