6BF9

Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 7.2 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation 3D Report Full Report


This is version 1.1 of the entry. See complete history

Literature

Ensemble cryo-EM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Zhang, Z.Liang, G.W.Bailey, J.L.Tan, Y.Z.Wei, H.McCord, A.L.Woods, A.Farcasanu, M.Wang, A.Lee, D.Shang, W.Meredith, C.S.Deprez-Poulain, R.Deprez, B.Liu, D.Koide, S.Lissiakoff, A.A.Li, S.Carraghter, B.Potter, S.C.Tang, W.J.

To be published.

Macromolecules

Find similar proteins by: Sequence  |  Structure

Entity ID: 1
MoleculeChainsSequence LengthOrganismDetails
Insulin-degrading enzyme
A, B
966Homo sapiensGene Names: IDE
EC: 3.4.24.56
Find proteins for P14735 (Homo sapiens)
Go to Gene View: IDE
Go to UniProtKB:  P14735
Entity ID: 2
MoleculeChainsSequence LengthOrganismDetails
Fab H11-E heavy chain
C, E
218Homo sapiensN/A
Find proteins for P0DOX5 (Homo sapiens)
Go to UniProtKB:  P0DOX5
Entity ID: 3
MoleculeChainsSequence LengthOrganismDetails
Fab H11-E light chain
D, F
211Homo sapiensN/A
Find proteins for P0DOX7 (Homo sapiens)
Go to UniProtKB:  P0DOX7
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 7.2 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
Software Package:
Software NamePurpose
PHENIXrefinement

Structure Validation

View Full Validation Report or Ramachandran Plots



Entry History & Funding Information

Deposition Data


Funding OrganizationCountryGrant Number
National Institutes of Health/National Institute of General Medical SciencesUnited StatesGM103310
National Institutes of Health/National Institute of General Medical SciencesUnited StatesGM81539
National Institutes of Health/National Institute of General Medical SciencesUnited StatesGM121964
Simons FoundationUnited States349247

Revision History 

  • Version 1.0: 2018-02-07
    Type: Initial release
  • Version 1.1: 2018-02-14
    Type: Author supporting evidence