6GYU

Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation 3D Report Full Report


This is version 1.0 of the entry. See complete history

Literature

Architecture of the CBF3-centromere complex of the budding yeast kinetochore.

Yan, K.Zhang, Z.Yang, J.McLaughlin, S.H.Barford, D.

(2018) Nat. Struct. Mol. Biol. --: --


Macromolecules

Find similar proteins by: Sequence  |  Structure

Entity ID: 1
MoleculeChainsSequence LengthOrganismDetails
Centromere DNA-binding protein complex CBF3 subunit B
B
608Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Mutation(s): 0 
Gene Names: CEP3 (CBF3, CBF3B, CSL1)
Find proteins for P40969 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Go to UniProtKB:  P40969
Entity ID: 2
MoleculeChainsSequence LengthOrganismDetails
Centromere DNA-binding protein complex CBF3 subunit C
A
478Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Mutation(s): 0 
Gene Names: CTF13 (CBF3C)
Find proteins for P35203 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Go to UniProtKB:  P35203
Entity ID: 3
MoleculeChainsSequence LengthOrganismDetails
Centromere DNA-binding protein complex CBF3 subunit B
C
564Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Mutation(s): 0 
Gene Names: CEP3 (CBF3, CBF3B, CSL1)
Find proteins for P40969 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Go to UniProtKB:  P40969
Entity ID: 4
MoleculeChainsSequence LengthOrganismDetails
Suppressor of kinetochore protein 1
D
194Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Mutation(s): 0 
Gene Names: SKP1 (CBF3D)
Find proteins for P52286 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Go to Gene View: SKP1
Go to UniProtKB:  P52286
Entity ID: 5
MoleculeChainsSequence LengthOrganismDetails
Centromere DNA-binding protein complex CBF3 subunit A
E
956Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Mutation(s): 0 
Gene Names: CBF2 (CBF3A, CEP2, CTF14, NDC10)
Find proteins for P32504 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Go to UniProtKB:  P32504
Small Molecules
Ligands 1 Unique
IDChainsName / Formula / InChI Key2D Diagram3D Interactions
ZN
Query on ZN

Download SDF File 
Download CCD File 
B
ZINC ION
Zn
PTFCDOFLOPIGGS-UHFFFAOYSA-N
 Ligand Interaction
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
Software Package:
Software NamePurpose
PHENIXrefinement
phenix.real_space_refinerefinement
phenix.real_space_refinerefinement
PHENIXrefinement

Structure Validation

View Full Validation Report or Ramachandran Plots



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Medical Research Council (United Kingdom)United KingdomMRC_UP_1201/6
Cancer Research UKUnited KingdomC576/A14109

Revision History 

  • Version 1.0: 2018-12-05
    Type: Initial release