6N1P

Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.35 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation 3D Report Full Report


This is version 1.0 of the entry. See complete history

Literature

CryoEM structures of open dimers of Gyrase A in complex with DNA illuminate mechanism of strand passage.

Soczek, K.M.Grant, T.Rosenthal, P.B.Mondragon, A.

(2018) Elife 7: --


Macromolecules

Find similar proteins by: Sequence  |  Structure


Entity ID: 1
MoleculeChainsSequence LengthOrganismDetails
DNA gyrase subunit A
A, B, C, D, E, F, G, H
511Streptococcus pneumoniaeMutation(s): 0 
Gene Names: gyrA_1 (gyrA)
EC: 5.99.1.3
Find proteins for A0A0Y2BJX7 (Streptococcus pneumoniae)
Go to UniProtKB:  A0A0Y2BJX7
Entity ID: 2
MoleculeChainsLengthOrganism
DNA (44-MER)I44Cloning vector pBR322
Entity ID: 3
MoleculeChainsLengthOrganism
DNA (44-MER)J44Cloning vector pBR322
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.35 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

View Full Validation Report or Ramachandran Plots



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Institutes of Health/National Institute of General Medical SciencesUnited StatesR01-GM051350
National Institutes of Health/National Institute of General Medical SciencesUnited StatesR35-GM118108
Cancer Research UKUnited KingdomFC001143
Medical Research Council (United Kingdom)United KingdomFC001143
Wellcome TrustUnited KingdomFC001143

Revision History 

  • Version 1.0: 2018-12-05
    Type: Initial release