6NBC

human methemoglobin state 1 determined using single-particle cryo-EM at 200 keV


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 2.8 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation 3D Report Full Report


This is version 1.1 of the entry. See complete history

Literature

High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.

Herzik Jr., M.A.Wu, M.Lander, G.C.

(2019) Nat Commun 10: 1032-1032

  • DOI: 10.1038/s41467-019-08991-8
  • Primary Citation of Related Structures:  

  • PubMed Abstract: 
  • Determining high-resolution structures of biological macromolecules amassing less than 100 kilodaltons (kDa) has been a longstanding goal of the cryo-electron microscopy (cryo-EM) community. While the Volta phase plate has enabled visualization of sp ...

    Determining high-resolution structures of biological macromolecules amassing less than 100 kilodaltons (kDa) has been a longstanding goal of the cryo-electron microscopy (cryo-EM) community. While the Volta phase plate has enabled visualization of specimens in this size range, this instrumentation is not yet fully automated and can present technical challenges. Here, we show that conventional defocus-based cryo-EM methodologies can be used to determine high-resolution structures of specimens amassing less than 100 kDa using a transmission electron microscope operating at 200 keV coupled with a direct electron detector. Our ~2.7 Å structure of alcohol dehydrogenase (82 kDa) proves that bound ligands can be resolved with high fidelity to enable investigation of drug-target interactions. Our ~2.8 Å and ~3.2 Å structures of methemoglobin demonstrate that distinct conformational states can be identified within a dataset for proteins as small as 64 kDa. Furthermore, we provide the sub-nanometer cryo-EM structure of a sub-50 kDa protein.


    Organizational Affiliation

    Griffith Institute for Drug Discovery, Griffith University, Nathan, Queensland, Australia.,College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, China.,Department of Chemistry, Boston University, Boston, MA 02215, USA.,Department of Bioengineering, James H. Clark Center, Stanford University, Stanford, CA 94305; shannon-au@cuhk.edu.hk wahc@stanford.edu.,Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, United States.,State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.,Department of Genetics, Harvard Medical School, Boston, United States.,Department of Biotechnology, Chemistry and Pharmacy, Department of Excellence 2018-2022, University of Siena, via Aldo Moro, 2, 53100 Siena (SI), Italy; Lead Discovery Siena S.r.l., Castelnuovo Berardenga, Siena, Italy; Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA, United States.,Centre for BioImaging Sciences, National University of Singapore, Singapore 117557.,Department of Chemistry , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.,Wound Infections Department, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.,State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Centre for Structural Biology, School of Life Sciences, Tsinghua University, 100084, Beijing, China. jwwang@mail.tsinghua.edu.cn.,Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA.,Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322.,Takeda California, 10410 Science Center Drive, San Diego, CA, 92121, USA.,Institute of Molecular Genetics, IGM-CNR, via Abbiategrasso 207, 27100 Pavia, Italy.,Institut für Biophysik, Johann Wolfgang von Goethe-Universität, Max-von-Laue-Str. 1, 60438, Frankfurt, Germany.,Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, MT 59812.,Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall Research Station, Vancouver, BC, V6T 1Z4, Canada.,Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany. beckmann@genzentrum.lmu.de.,From the Center for Structural Biology, Department of Biochemistry.,School of Chemistry and Biomedical Sciences Research Complex, University of St Andrews, Fife, Scotland, United Kingdom.,Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.,Structural Molecular Microbiology, Vlaams Instituut voor Biotechnologie (VIB), 1050 Brussels, Belgium. henri.de.greve@vub.be.,Department of Medical Genetics, University of Cambridge and Cambridge NIHR Biomedical Research Centre, Cambridge, UK.,Sorbonne Université, CNRS, Laboratoire de Réactivité de Surface (LRS), UPMC Univ Paris 06, UMR 7197, Paris, F-75005, France.,HIV Dynamics and Replication Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.,Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA, USA.,Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.,Laboratory of Medicinal Chemistry, Center for Interdisciplinary Research on Medicines (CIRM) , ULiège , Quartier Hôpital, Avenue Hippocrate, 15, B36 , B-4000 Liège , Belgium.,School of Life Science and Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro, Tokyo 152-8550, Japan. Electronic address: akitao@bio.titech.ac.jp.,Department of Chemistry and Biochemistry, University of Bern, 3012 Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.,Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium. serge.muyldermans@vub.be.,Department of Biotechnology, Chemistry and Pharmacy, Department of Excellence 2018-2022, University of Siena, via Aldo Moro, 2, 53100 Siena (SI), Italy.,Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551.,Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.,Center for Structural Genomics of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA e-filippova@northwestern.edu wf-anderson@northwestern.edu.,Department of Chemistry, Boston University, Boston, MA 02215, USA; Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, MA 02118, USA.,Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.,Division of Cardiovascular Medicine and the Gill Heart Institute, University of Kentucky, Lexington, KY, USA.,Universität Bayreuth, Lehrstuhl Biopolymere, Universitätsstr. 30, D-95447, Bayreuth, Germany.,Department of Cardiometabolic Disorders, Amgen Discovery Research, Amgen Inc., South San Francisco, California 94080 yangln3101@gmail.com.,School of Life Sciences, GIST, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.,Crop Science, Bayer AG, 40789 Monheim, Germany.,Molecular and Cell Biology, International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan.,Departments of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA, USA. kcgarcia@stanford.edu.,School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong.,Biozentrum, University of Basel, Basel, Switzerland.,Structural Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.,Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.,Emory Vaccine Center, School of Medicine, Emory University, Atlanta, Georgia, USA.,Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zürich, 8093 Zürich, Switzerland. Electronic address: allain@mol.biol.ethz.ch.,Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.,Universität Bayreuth, Lehrstuhl Biopolymere, Universitätsstr. 30, D-95447, Bayreuth, Germany. birgitta.woehrl@uni-bayreuth.de.,the Division of Hematology, Children's Hospital of Philadelphia, and the Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104.,Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: gaof@im.ac.cn.,School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong; shannon-au@cuhk.edu.hk wahc@stanford.edu.,Department of Chemistry, Boston University, Boston, MA 02215, USA. Electronic address: pinghua@bu.edu.,Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.,Laboratory of Molecular and Genetic Analysis, Center for Molecular Biology and Genetic Engineering, Institute of Biology, University of Campinas, CP6010, 13083-875 Campinas-SP, Brazil.,CIC bioGUNE, Parque Tecnológico de Bizkaia Edificio 800, 48160 Derio, Spain.,Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China.,Laboratory of Structural Biology and Crystallography, Institute of Chemistry, University of Campinas, CP6154, 13083-970 Campinas-SP, Brazil.,Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.,Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.,Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung, 80424, Taiwan.,Center for Integrated Protein Science at the Department Chemie, Technische Universitat München, Lichtenbergstraße 4, 85748 Garching, Germany.,Center for Structural Studies, Heinrich-Heine-University Düsseldorf, D-40225, Düsseldorf, Germany.,State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China.,Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.,CAS Centre for Excellence in Molecular Cell Science, University of Science and Technology of China, Chinese Academy of Sciences, 230027, Hefei, China. sunlf17@ustc.edu.cn.,Department of Pediatrics, Hadassah Hebrew University Medical Center, Jerusalem, Israel.,Department of Applied Biology, Dong-A University, Busan, Korea.,Institute of Structural Biology, Nanyang Technological University, Singapore 639798.,Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, London, UK. c.moores@mail.cryst.bbk.ac.uk.,Department of Cell Biology, Harvard Medical School, 250 Longwood Avenue, SGM 509, Boston, MA 02115, USA. david.mueller@rosalindfranklin.edu maofu_liao@hms.harvard.edu.,Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, Brasília, 70790-160, DF, Brazil.,the Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724.,Department of Bioengineering, Rice University, 6100 Main St., Houston, TX 77005, USA. Electronic address: jsuh@rice.edu.,GE Healthcare Life Sciences, Beijing 100176, China.,State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China.,Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium. joar.pinto@vub.be.,Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah Hebrew University Medical Center, Jerusalem, Israel.,Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.,Department of Microbiology and Immunology, Loyola University Chicago, Health Sciences Division, Stritch School of Medicine, Maywood, Illinois, USA.,LOEWE Center for Synthetic Microbiology, Philipps-University Marburg, D-35043, Marburg, Germany.,Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. glander@scripps.edu.,Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY, USA. ruta@rockefeller.edu.,From the Department of Therapeutic Discovery and zwang@amgen.com.,Department of Biological Chemistry, University of Michigan Medical School, 1150 W. Medical Center Drive, SPC 5606, Ann Arbor, MI 48109, USA.,Institute of Biochemistry, Heinrich-Heine-University Düsseldorf, D-40225, Düsseldorf, Germany. sander.smits@hhu.de.,Forschungszentrum für Bio-Makromoleküle, Universitätsstr. 30, D-95447, Bayreuth, Germany.,Service of Infectious Diseases, University Hospital Geneva, Geneva, Switzerland thilo.kohler@unige.ch.,Pohang Accelerator Laboratory, Pohang, Gyeongbuk 37673, Republic of Korea.,Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville Melbourne, VIC, Australia.,Institute of Molecular Biology, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 11529, Taiwan.,Universität Bayreuth, Lehrstuhl Biopolymere, Universitätsstr. 30, D-95447, Bayreuth, Germany. stefan.knauer@uni-bayreuth.de.,Biological Science Imaging facility (BSIR), Department of Biology, 89 Chieftan Way, Florida State University, Tallahassee, FL 32306, USA.,Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, Departments of Biological Chemistry and Physiology, and UCLA-DOE Institute, University of California, Los Angeles, California 90095, United States.,S-Inova Biotech, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, 79117-010, MS, Brazil. ocfranco@gmail.com.,Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada. Electronic address: alexei.savchenko@ucalgary.ca.,Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA. kcgarcia@stanford.edu.,Department of Chemistry, Analytical Chemistry, Philipps-University Marburg, D-35043, Marburg, Germany.,Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan. Electronic address: kimada@chem.sci.osaka-u.ac.jp.,School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.,Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.,Service of Infectious Diseases, University Hospital Geneva, Geneva, Switzerland.,Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden.,Biosciences Division, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025.,Department of Biological Chemistry and Molecular Biology, Chicago Medical School, Rosalind Franklin University, 3333 Green Bay Road, North Chicago, IL 60064, USA. david.mueller@rosalindfranklin.edu maofu_liao@hms.harvard.edu.,Laboratory of Drug Design and Medicinal Chemistry, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan.,Department of Biological Sciences, Virginia Tech, Derring Hall, Blacksburg, VA 24061, U.S.A.,Department of Structural & Chemical Biology, Centro de Investigaciones Biológicas, CIB-CSIC 28040 Madrid, Spain. Electronic address: earias@cib.csic.es.,Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.,Medical Research Council Laboratory of Molecular Biology, Cambridge, UK damgaard@mrc-lmb.cam.ac.uk dkomander@wehi.edu.au berkun@hadassah.org.il.,Molecular Pathology Post-graduate Program, University of Brasília, Brasília, 70.910-900, DF, Brazil. ocfranco@gmail.com.,Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; RIKEN Center for Biosystems Dynamics Research & SPring-8 Center, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.,Laboratório de RMN, Instituto de Química, Universidade Federal de Goiás, Goiânia, 74690-900, GO, Brazil.,Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium. yann.sterckx@vub.be.,Jacobs University, Bremen, Germany.,New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Republic of Korea.,Laboratory of Molecular Pharmacology, GIGA-Molecular Biology of Diseases , ULiège , B34, Quartier Hôpital, Avenue de l'hôpital, 11 , B-4000 Liège , Belgium.,Department of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA, USA.,Center for Redox Biology and Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157 and.,Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: tgonen@ucla.edu.,School of Life Sciences, Sun Yat-Sen University, 510275 Guangzhou, China.,LOEWE Center for Synthetic Microbiology, Philipps-University Marburg, D-35043, Marburg, Germany. bremer@staff.uni-marburg.de.,Department of Biochemistry and Molecular Biology, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.,Department of Chemistry , Emory University , Atlanta , Georgia 30322 , United States.,Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, Brasília, 70790-160, DF, Brazil. ocfranco@gmail.com.,Department of Biochemistry , Emory University , Atlanta , Georgia 30322 , United States.,School of Life Sciences, GIST, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea; Advanced Photonics Research Institute, GIST, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.,Department of Biochemistry, Virginia Tech, Engel Hall, Blacksburg, VA 24061, U.S.A.,Department of Chemistry and Biochemistry, University of Bern, 3012 Bern, Switzerland.,School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.,Institut für Biologie, Strukturbiologie/Biochemie, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099, Berlin, Germany.,Hebrew University Hadassah Medical School, Jerusalem, Israel.,Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.,Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China. sunlf17@ustc.edu.cn.,Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.,Center for Structural Studies, Heinrich-Heine-University Düsseldorf, D-40225, Düsseldorf, Germany. sander.smits@hhu.de.,Synthetic Biology Group, MIT Synthetic Biology Center; The Center for Microbiome Informatics and Therapeutics; Research Laboratory of Electronics, Department of Biological Engineering, and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, 02139, MA, USA.,Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia roisin.mcmahon@griffith.edu.au pmireland@dstl.gov.uk jlm@griffith.edu.au.,Institut für Virologie, Philipps-Universität, Robert-Koch-Strasse 17, Marburg 35037, Germany.,Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 6767 Bertner Avenue, Houston, TX 77225, USA.,Broad Institute of MIT and Harvard, Cambridge, 02139, MA, USA. cfuente@mit.edu.,Marshall Centre for Infectious Disease Research and Training, School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia, Australia.,Institute of Biological Chemistry, Washington State University, Pullman, Washington 99163.,Center for Molecular Signaling, Wake Forest University, Winston-Salem, North Carolina 27101.,Agricultural Solutions, BASF, Morrisville, NC 27560; Jelena.zaitseva@agro.basf-se.com.,Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zürich, 8093 Zürich, Switzerland; Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, 3800 VIC, Australia. Electronic address: fionna.loughlin@monash.edu.,Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, London, UK. s.manka@mail.cryst.bbk.ac.uk.,Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.,Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore 138673.,Department of Immunology and Microbiology, Scripps Research, La Jolla, California, USA.,Department of Cardiometabolic Disorders, Amgen Discovery Research, Amgen Inc., South San Francisco, California 94080.,College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, Jiangsu, China. Electronic address: zhanggaiping2003@163.com.,Department of Biochemistry and Center of Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.,Department of Biochemistry, University of Toronto, Toronto, ON, Canada.,Department of Cell Biology, Harvard Medical School, 250 Longwood Avenue, SGM 509, Boston, MA 02115, USA.,Department of Chemistry, University of Manitoba, 44 Dysart Rd, R3T 2N2, Winnipeg, MB, Canada.,Defence Science and Technology Laboratory, Chemical, Biological and Radiological Division, Porton Down, Salisbury, Wiltshire, United Kingdom roisin.mcmahon@griffith.edu.au pmireland@dstl.gov.uk jlm@griffith.edu.au.,Keck Biophysics Facility, Northwestern University, Evanston, Illinois, USA.,College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China.,Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA.,Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium. henri.de.greve@vub.be.,Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.,Department of Molecular Biology, Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, United States.,Department of Biological Sciences, Virginia Tech, Derring Hall, Blacksburg, VA 24061, U.S.A. bscharf@vt.edu fschubot@vt.edu.,Broad Institute of MIT and Harvard, Cambridge, 02139, MA, USA.,Department of Biology, Laboratory for Microbiology, Philipps-University Marburg, D-35043, Marburg, Germany.,Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zürich, 8093 Zürich, Switzerland.,Department of Biological Chemistry, University of Michigan Medical School, 1150 W. Medical Center Drive, SPC 5606, Ann Arbor, MI 48109, USA; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: uhnsoo@med.umich.edu.,Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, Houston, TX 77005, USA.,School of Biological Sciences, Nanyang Technological University, Singapore 637551; ygao@ntu.edu.sg yangliang@ntu.edu.sg rero@ntu.edu.sg.,Agricultural Solutions, BASF, Morrisville, NC 27560.,Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institut für Biochemie (CC2), Charitéplatz 1, D-10117 Berlin, Germany.,Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.,Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland.,Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Str. 10, 10623, Berlin, Germany.,The Hospital for Sick Children Research Institute and Department of Biochemistry, University of Toronto, Toronto, ON, Canada.,HIV Dynamics and Replication Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA hughesst@mail.nih.gov.,Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey, USA.,Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, NM 87801, USA; Department of Biology, New Mexico Institute of Mining and Technology, Socorro, NM 87801, USA.,Structural Genomics Consortium, University of Toronto, Toronto, ON, Canada; Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.,CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025.,Pacific Northwest National Laboratory, Richland, Washington 99352, and.,Department of Biochemistry, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.,Department of Bioengineering, Rice University, 6100 Main St., Houston, TX 77005, USA.,Biostructural Research, Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences , University of Copenhagen , Universitetsparken 2 , DK-2100 Copenhagen , Denmark.,Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, Egypt.,Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78712, USA.,Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: tohru@fbs.osaka-u.ac.jp.,Department of Biological Chemistry, University of Michigan Medical School, 1150 W. Medical Center Drive, SPC 5606, Ann Arbor, MI 48109, USA; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA.,Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA.,Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.,State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.,Porto Reports, Brasília, 72236-011, DF, Brazil.,Emory Vaccine Center, School of Medicine, Emory University, Atlanta, Georgia, USA florian.krammer@mssm.edu ellebedy@wustl.edu.,Institute of Biochemistry II, Goethe University, Frankfurt am Main 60590, Germany.,Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD, USA.,Doctoral Degree Program in Marine Biotechnology, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 11529, Taiwan.,Hebrew University Hadassah Medical School, Jerusalem, Israel damgaard@mrc-lmb.cam.ac.uk dkomander@wehi.edu.au berkun@hadassah.org.il.,Defence Science and Technology Laboratory, Chemical, Biological and Radiological Division, Porton Down, Salisbury, Wiltshire, United Kingdom.,Department of Biochemistry , University of Bayreuth , Universitätsstraße 30 , 95445 Bayreuth , Germany.,Novartis Institutes for BioMedical Research, Novartis Campus, 4002, Basel, Switzerland.,Department of Biology, Laboratory for Microbiology, Philipps-University Marburg, D-35043, Marburg, Germany. bremer@staff.uni-marburg.de.,The Warren and Katharine Schlinger Laboratory of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.,Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, NM 87801, USA. Electronic address: rodolfo.tello-aburto@nmt.edu.,Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland.,CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.,Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany.,Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester, Lancaster Rd, Leicester LE1 7HB, UK.,Department of Medical Biology, The University of Melbourne, Melbourne VIC, Australia.,Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, São Paulo, 09210-580, Brazil.,State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. Electronic address: chenlei2016@pku.edu.cn.,S-Inova Biotech, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, 79117-010, MS, Brazil.,Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.,Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China.,Department of Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.,National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.,Monique and Jacques Roboh Department of Genetic Research, Hadassah Hebrew University Medical Center, Jerusalem, Israel.,IKERBASQUE, Basque Foundation for Science, Bilbao, Spain.,Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: jmberger@jhmi.edu.,Pacific Northwest National Laboratory, Richland, Washington 99352, and lance.seefeldt@usu.edu.,Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.,Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium. ema.estevens.romao@vub.be.,Global Discovery Chemistry, Novartis Institutes for BioMedical Research, Novartis Campus, 4002, Basel, Switzerland.,Crop Science, Bayer US, Durham, NC 27703.,Department of Bioengineering, James H. Clark Center, Stanford University, Stanford, CA 94305.,Laboratory of Bacterial Genetics, Institute of Biology, University of Campinas (UNICAMP), São Paulo 13083-862, Brazil. tyano@unicamp.br.,Department of Immunology and Microbiology, Scripps Research, La Jolla, California, USA erica@scripps.edu.,Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK. Electronic address: d.kelly@sheffield.ac.uk.,Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institut für Medizinische Physik und Biophysik (CC2), Group Protein X-ray Crystallography and Signal Transduction, Charitéplatz 1, D-10117 Berlin, Germany. Electronic address: patrick.scheerer@charite.de.,Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA. jennyh@niddk.nih.gov.,Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, USA.,Department of Pathology, School of Medicine, Stanford University, Palo Alto, CA, USA.,Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited, 26-1, Muraoka-higashi 2-chome, Fujisawa, Kanagawa, 251-8555, Japan.,Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA. nkorotkova@uky.edu.,Laboratory of Drug Design and Medicinal Chemistry, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan. Electronic address: titoh@ac.shoyaku.ac.jp.,Stanford Blood Center, Palo Alto, CA, USA.,Laboratory of Bacterial Genetics, Institute of Biology, University of Campinas (UNICAMP), São Paulo 13083-862, Brazil. alvinbn@gmail.com.,From the School of Life and Environmental Sciences, University of Sydney New South Wales 2006, Australia, joel.mackay@sydney.edu.au.,NAmur MEdicine & Drug Innovation Center (NAMEDIC), NARILIS, UNamur , rue de Bruxelles 61 , B-5000 Namur , Belgium.,Department of Microbiology and Immunology, Columbia University, New York, NY, USA.,From the Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, jw.peters@wsu.edu.,Institute of Advanced Chemistry of Catalonia (IQAC), CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.,Institute of Chemical Biology and Drug Discovery and Department of Chemistry, Stony Brook University, Stony Brook, NY 11794-3400, USA.,Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA.,Biosyntia ApS, Copenhagen, 2100, Denmark.,Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Biogenèse des Signaux Peptidiques (BIOSIPE), UPMC Univ Paris 06, Paris, F-75005, France.,Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany. becker@genzentrum.lmu.de.,Molecular Pathology Post-graduate Program, University of Brasília, Brasília, 70.910-900, DF, Brazil.,From the Department of Therapeutic Discovery and xmin@amgen.com.,Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.,Structural Biology Center, Biosciences Division, Argonne National Laboratory, Argonne, IL, USA.,Department of Biotechnology, Chemistry and Pharmacy, Department of Excellence 2018-2022, University of Siena, via Aldo Moro, 2, 53100 Siena (SI), Italy. Electronic address: pozzi4@unisi.it.,Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany. beatrix@genzentrum.lmu.de.,College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China.,Present address: Hospira Zagreb d.o.o., a Pfizer company, Prudnicka cesta 60, 10291, Prigorje Brdovecko, Croatia.,From the Department of Therapeutic Discovery and.,Department of Medicine, Section of Rheumatology, The Committee on Immunology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA.,Institute for Molecular Engineering and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA.,Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.,Department of Cell Biology & Molecular Genetics and Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.,From the School of Life and Environmental Sciences, University of Sydney New South Wales 2006, Australia.,Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.,Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA.,Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, NM 87801, USA.,School of Life Sciences, GIST, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: misunjin@gist.ac.kr.,Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.,Division of Biophysics, Department of Biology, University of Osnabruck, Osnabruck, Germany.,the Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand, and.,Rega Institute for Medical Research and Department of Microbiology and Immunology, Leuven, Belgium.,Kekulé-Institute for Organic Chemistry and Biochemistry, Friedrich-Wilhelms-University Bonn, D-53121, Bonn, Germany.,Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands. nsorge3@umcutrecht.nl.,Molecular Biotechnology Group, Department of Physics and Interdisciplinary Science, Sao Carlos Institute of Physics (IFSC), University of Sao Paulo (USP), Avenida Trabalhador São-carlense 400, Parque Arnold Schimidt, 13566-590 São Carlos-SP, Brazil.,Laboratory of Molecular Electron Microscopy, The Rockefeller University, New York, NY, USA.,Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158.,Department of Biochemistry , Brandeis University , Waltham , Massachusetts 02454 , United States.,The Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.,Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA florian.krammer@mssm.edu ellebedy@wustl.edu.,CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.,Skaggs Institute for Chemical Biology, Scripps Research, La Jolla, California, USA.,Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.,Department of Biological Chemistry and Molecular Biology, Chicago Medical School, Rosalind Franklin University, 3333 Green Bay Road, North Chicago, IL 60064, USA.,Structural Biology Laboratory, Elettra-Sincrotrone Trieste S.C.p.A., Trieste 34149, Italy.,School of Biological Sciences, Nanyang Technological University, Singapore 637551.,Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY, USA.,Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.,Donnelly Centre for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, M5S 3E1 Toronto, Ontario, Canada.,Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.,Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.,Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institut für Medizinische Physik und Biophysik (CC2), Group Protein X-ray Crystallography and Signal Transduction, Charitéplatz 1, D-10117 Berlin, Germany.,Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, Georgia, USA.,Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institut für Biochemie (CC2), Charitéplatz 1, D-10117 Berlin, Germany. Electronic address: hartmut.kuhn@charite.de.,Section on Molecular Medicine, Department of Internal Medicine.,From the Center for Structural Biology, Department of Biochemistry, tlowther@wakehealth.edu.,National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA.,Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey, USA.,Department of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.,Synthetic Biology Group, MIT Synthetic Biology Center; The Center for Microbiome Informatics and Therapeutics; Research Laboratory of Electronics, Department of Biological Engineering, and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, 02139, MA, USA. cfuente@mit.edu.,Theoretical Molecular Biophysics Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA.,Department of Biology, New Mexico Institute of Mining and Technology, Socorro, NM 87801, USA.,Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.,Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.,Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA. Electronic address: jzhang@cm.utexas.edu.,Departments of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.,Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.,From the Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717.,Callaghan Innovation, Gracefield, Lower Hutt, New Zealand.,Université Côte d'Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne Sophia Antipolis, France.,Wake Forest Baptist Comprehensive Cancer Center, and.




Macromolecules

Find similar proteins by: Sequence  |  Structure

Entity ID: 1
MoleculeChainsSequence LengthOrganismDetails
Hemoglobin subunit alpha
A, C
140Homo sapiensMutation(s): 0 
Gene Names: HBA1, HBA2
Find proteins for P69905 (Homo sapiens)
Go to Gene View: HBA1 HBA2
Go to UniProtKB:  P69905
Entity ID: 2
MoleculeChainsSequence LengthOrganismDetails
Hemoglobin subunit beta
B, D
143Homo sapiensMutation(s): 0 
Gene Names: HBB
Find proteins for P68871 (Homo sapiens)
Go to Gene View: HBB
Go to UniProtKB:  P68871
Small Molecules
Ligands 1 Unique
IDChainsName / Formula / InChI Key2D Diagram3D Interactions
HEM
Query on HEM

Download SDF File 
Download CCD File 
A, B, C, D
PROTOPORPHYRIN IX CONTAINING FE
HEME
C34 H32 Fe N4 O4
KABFMIBPWCXCRK-RGGAHWMASA-L
 Ligand Interaction
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 2.8 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
Software Package:
Software NamePurpose
PHENIXrefinement

Structure Validation

View Full Validation Report or Ramachandran Plots



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Institutes of Health/National Human Genome Research InstituteUnited StatesDP2EB020402

Revision History 

  • Version 1.0: 2019-03-13
    Type: Initial release
  • Version 1.1: 2019-03-20
    Type: Data collection, Database references