5IIT

Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1

Present annotations:

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Product Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Macro domain-like	8036145	3000458	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Macro domain-like	8036145	3000458	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Macro domain-like	8036145	3000458	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Macro domain-like	8036145	3000458	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SPX	e5iitA2	A: alpha bundles	X: SPX domain (From Topology)	H: SPX domain (From Topology)	T: SPX domain	F: SPX	ECOD (1.6)
A	Macro	e5iitA1	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro	ECOD (1.6)
B	SPX	e5iitB2	A: alpha bundles	X: SPX domain (From Topology)	H: SPX domain (From Topology)	T: SPX domain	F: SPX	ECOD (1.6)
B	Macro	e5iitB1	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro	ECOD (1.6)
C	SPX	e5iitC2	A: alpha bundles	X: SPX domain (From Topology)	H: SPX domain (From Topology)	T: SPX domain	F: SPX	ECOD (1.6)
C	Macro	e5iitC1	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro	ECOD (1.6)
D	SPX	e5iitD2	A: alpha bundles	X: SPX domain (From Topology)	H: SPX domain (From Topology)	T: SPX domain	F: SPX	ECOD (1.6)
D	Macro	e5iitD1	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF01661	Macro domain (Macro)	Macro domain	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently ...	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently [2]; while in other cases (such as Aprataxin) it appears to bind both non-covalently through a zinc finger motif, and covalently through a separate region of the protein [3]. This domain is found in a number of otherwise unrelated proteins. It is found at the C-terminus of the macro-H2A histone protein 4 and also in the non-structural proteins of several types of ssRNA viruses such as NSP3 from alpha-viruses and coronaviruses. This domain is also found on its own in a family of proteins from bacteria, archaebacteria and eukaryotes. The 3D structure of the SARS-CoV Macro domain has a mixed alpha/beta fold consisting of a central seven-stranded twisted mixed beta sheet sandwiched between two alpha helices on one face, and three on the other. The final alpha-helix, located on the edge of the central beta-sheet, forms the C terminus of the protein [4]. The crystal structure of AF1521 (a Macro domain-only protein from Archaeoglobus fulgidus) has also been reported and compared with other Macro domain containing proteins. Several Macro domain only proteins are shorter than AF1521, and appear to lack either the first strand of the beta-sheet or the C-terminal helix 5. Well conserved residues form a hydrophobic cleft and cluster around the AF1521-ADP-ribose binding site [5].	Domain

Gene Product Annotation Gene Product Annotation

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Vacuolar transporter chaperone 4,Core histone macro-H2A.1	kinase activity transferase activity polyphosphate kinase activity catalytic activity phosphotransferase activity, phosphate group as acceptor transferase activity, transferring phosphorus-containing groups protein binding binding calmodulin binding ion binding inositol hexakisphosphate binding alcohol binding anion binding small molecule binding kinase activity transferase activity polyphosphate kinase activity catalytic activity phosphotransferase activity, phosphate group as acceptor transferase activity, transferring phosphorus-containing groups protein binding binding calmodulin binding ion binding inositol hexakisphosphate binding alcohol binding anion binding small molecule binding DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding heterocyclic compound binding ADP-D-ribose binding carbohydrate derivative binding protein-containing complex binding chromatin binding promoter-specific chromatin binding rDNA binding double-stranded methylated DNA binding protein heterodimerization activity protein dimerization activity structural molecule activity structural constituent of chromatin RNA polymerase II transcription regulatory region sequence-specific DNA binding kinase binding enzyme binding protein kinase binding chromatin DNA binding ADP-D-ribose modification-dependent protein binding poly-ADP-D-ribose modification-dependent protein binding modification-dependent protein binding nucleosome binding nucleosomal DNA binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity	cellular catabolic process lysosomal microautophagy catabolic process cellular metabolic process autophagy cellular process metabolic process process utilizing autophagic mechanism localization cellular localization transport establishment of localization in cell vacuolar transport intracellular transport cellular catabolic process lysosomal microautophagy catabolic process cellular metabolic process autophagy cellular process metabolic process process utilizing autophagic mechanism localization cellular localization transport establishment of localization in cell vacuolar transport intracellular transport establishment of localization organelle fusion cellular component organization vacuole fusion organelle organization vacuole organization vacuole fusion, non-autophagic cellular component organization or biogenesis membrane organization engulfment of target by autophagosome autophagosome assembly organelle assembly macroautophagy autophagosome organization cellular component biogenesis cellular component assembly membrane invagination oxoacid metabolic process cellular biosynthetic process organic acid metabolic process polyphosphate metabolic process organic substance metabolic process biosynthetic process polyphosphate biosynthetic process small molecule metabolic process phosphorus metabolic process response to oxidative stress regulation of biological process response to stimulus regulation of response to stimulus response to stress regulation of response to stress regulation of response to oxidative stress biological regulation regulation of cellular process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process protein-DNA complex organization positive regulation of metabolic process positive regulation of biosynthetic process chromatin organization organic substance biosynthetic process chromatin remodeling positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process protein-containing complex organization epigenetic regulation of gene expression regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression positive regulation of gene expression transcription initiation-coupled chromatin remodeling regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process negative regulation of response to stimulus negative regulation of biological process negative regulation of response to oxidative stress aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds regulation of generation of precursor metabolites and energy generation of precursor metabolites and energy regulation of cellular respiration regulation of aerobic respiration regulation of oxidative phosphorylation cellular response to stimulus cellular nitrogen compound metabolic process primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process heterocycle metabolic process cellular response to stress DNA damage response nitrogen compound metabolic process cellular aromatic compound metabolic process DNA repair negative regulation of nitrogen compound metabolic process cellular nitrogen compound biosynthetic process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process regulation of nitrogen compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process negative regulation of cellular biosynthetic process aromatic compound biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process RNA metabolic process RNA biosynthetic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process heterocycle biosynthetic process negative regulation of biosynthetic process negative regulation of transcription of nucleolar large rRNA by RNA polymerase I regulation of ncRNA transcription transcription by RNA polymerase I rRNA transcription regulation of transcription by RNA polymerase I regulation of transcription of nucleolar large rRNA by RNA polymerase I negative regulation of transcription by RNA polymerase I rRNA metabolic process nucleolar large rRNA transcription by RNA polymerase I ncRNA transcription ncRNA metabolic process dosage compensation cell cycle G2/M phase transition regulation of cell cycle phase transition regulation of cell cycle process negative regulation of cell cycle regulation of cell cycle G2/M phase transition cell cycle phase transition negative regulation of cell cycle process negative regulation of cell cycle G2/M phase transition negative regulation of cell cycle phase transition cell cycle regulation of cell cycle cell cycle process regulation of lipid metabolic process lipid metabolic process regulation of NAD metabolic process NAD metabolic process purine nucleotide metabolic process regulation of small molecule metabolic process nucleobase-containing small molecule metabolic process regulation of nucleotide metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process organonitrogen compound metabolic process regulation of purine nucleotide metabolic process nucleotide metabolic process purine-containing compound metabolic process organophosphate metabolic process regulation of phosphorus metabolic process regulation of maintenance of mitotic sister chromatid cohesion positive regulation of organelle organization regulation of cellular component organization positive regulation of cellular component organization mitotic sister chromatid cohesion maintenance of sister chromatid cohesion maintenance of mitotic sister chromatid cohesion positive regulation of cell cycle process chromosome organization sister chromatid cohesion regulation of chromosome organization positive regulation of maintenance of sister chromatid cohesion positive regulation of maintenance of mitotic sister chromatid cohesion positive regulation of sister chromatid cohesion regulation of organelle organization positive regulation of cell cycle positive regulation of chromosome organization regulation of maintenance of sister chromatid cohesion regulation of sister chromatid cohesion positive regulation of response to oxidative stress positive regulation of response to stimulus protein localization regulation of localization regulation of protein localization negative regulation of protein localization to chromosome, telomeric region protein localization to chromosome, telomeric region regulation of protein localization to chromosome, telomeric region protein localization to chromosome regulation of cellular localization cellular macromolecule localization macromolecule localization protein localization to organelle negative regulation of protein localization negative regulation of gene expression negative regulation of gene expression, epigenetic protein-DNA complex assembly protein-containing complex assembly nucleosome assembly nucleosome organization multicellular organismal process regulation of developmental process cellular developmental process regulation of cell differentiation embryo development positive regulation of developmental process multicellular organism development positive regulation of cell differentiation regulation of endodermal cell differentiation cell differentiation anatomical structure development developmental process formation of primary germ layer embryonic morphogenesis positive regulation of endodermal cell differentiation tissue development gastrulation anatomical structure morphogenesis endoderm development anatomical structure formation involved in morphogenesis endoderm formation endodermal cell differentiation regulation of catalytic activity negative regulation of catalytic activity regulation of transferase activity negative regulation of kinase activity negative regulation of transferase activity negative regulation of protein modification process protein modification process negative regulation of protein metabolic process protein phosphorylation phosphorylation regulation of protein metabolic process regulation of molecular function regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification regulation of protein serine/threonine kinase activity regulation of protein modification process regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of protein serine/threonine kinase activity protein metabolic process negative regulation of molecular function negative regulation of protein kinase activity regulation of protein kinase activity negative regulation of phosphorus metabolic process regulation of phosphorylation negative regulation of phosphorylation positive regulation of keratinocyte differentiation regulation of epidermal cell differentiation epidermis development epithelial cell differentiation regulation of epithelial cell differentiation regulation of multicellular organismal process epithelium development regulation of multicellular organismal development epidermal cell differentiation animal organ development positive regulation of epidermis development positive regulation of epithelial cell differentiation skin development keratinocyte differentiation positive regulation of multicellular organismal process regulation of epidermis development regulation of keratinocyte differentiation positive regulation of epidermal cell differentiation establishment of protein localization protein localization to chromatin establishment of protein localization to chromatin establishment of protein localization to organelle establishment of protein localization to chromosome	membrane-bounded organelle intracellular organelle intracellular anatomical structure cytoplasmic vesicle organelle intracellular vesicle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle vesicle vacuolar membrane storage vacuole membrane lytic vacuole membrane-bounded organelle intracellular organelle intracellular anatomical structure cytoplasmic vesicle organelle intracellular vesicle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle vesicle vacuolar membrane storage vacuole membrane lytic vacuole vacuole fungal-type vacuole lytic vacuole membrane bounding membrane of organelle organelle membrane fungal-type vacuole membrane autophagosome membrane autophagosome cell periphery cell cortex protein-containing complex vacuolar transporter chaperone complex membrane protein complex intracellular protein-containing complex endoplasmic reticulum endomembrane system organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space extracellular membrane-bounded organelle protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle nucleus membrane-enclosed lumen nuclear chromosome intracellular organelle lumen organelle lumen nuclear lumen chromosome, telomeric region chromosomal region nucleolus condensed chromosome sex chromosome heterochromatin sex chromatin nucleosome Barr body X chromosome nucleoplasm pericentric heterochromatin chromosome, centromeric region site of DNA damage

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.