ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg35.159
r_dihedral_angle_3_deg19.34
r_dihedral_angle_1_deg14.769
r_dihedral_angle_4_deg13.54
r_mcangle_it5.272
r_mcbond_other2.758
r_mcbond_it2.757
r_angle_other_deg1.234
r_angle_refined_deg1.173
r_chiral_restr0.393
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg35.159
r_dihedral_angle_3_deg19.34
r_dihedral_angle_1_deg14.769
r_dihedral_angle_4_deg13.54
r_mcangle_it5.272
r_mcbond_other2.758
r_mcbond_it2.757
r_angle_other_deg1.234
r_angle_refined_deg1.173
r_chiral_restr0.393
r_gen_planes_refined0.007
r_bond_refined_d0.006
r_bond_other_d0.003
r_gen_planes_other0.003
Sample
Kluyveromyces lactis small ribosomal subunit in complex with the Cricket paralysis virus IRES
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK II
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles54481
Reported Resolution (Å)3.8
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Map-Model Fitting and Refinement
Id1
Refinement SpaceRECIPROCAL
Refinement ProtocolOTHER
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)25
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder ModelFEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONEPU
IMAGE ACQUISITIONEPU1.8
CTF CORRECTIONCTFFIND
INITIAL EULER ASSIGNMENTRELION1.4
MODEL REFINEMENTREFMAC5.8
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING ONLY