4UER

40S-eIF1-eIF1A-eIF3-eIF3j translation initiation complex from Lachancea kluyveri


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.47 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 2.0 of the entry. See complete history


Literature

Structure of a Yeast 40S-Eif1-Eif1A-Eif3-Eif3J Initiation Complex

Aylett, C.H.S.Boehringer, D.Erzberger, J.P.Schaefer, T.Ban, N.

(2015) Nat Struct Mol Biol 22: 269

  • DOI: 10.1038/nsmb.2963
  • Primary Citation of Related Structures:  
    4UER

  • PubMed Abstract: 
  • Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c ...

    Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.


    Organizational Affiliation

    Institute for Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.



Macromolecules

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Entity ID: 1
MoleculeChainsSequence LengthOrganismDetailsImage
EIF1AA [auth 0]100Lachancea kluyveriMutation(s): 0 
Find proteins for A0A0J9X202 (Lachancea kluyveri)
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Entity ID: 2
MoleculeChainsSequence LengthOrganismDetailsImage
ES28B [auth 1]63Lachancea kluyveriMutation(s): 0 
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Entity ID: 3
MoleculeChainsSequence LengthOrganismDetailsImage
ES8C [auth 2]188Lachancea kluyveriMutation(s): 0 
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Entity ID: 4
MoleculeChainsSequence LengthOrganismDetailsImage
ES7D [auth 3]184Lachancea kluyveriMutation(s): 0 
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Entity ID: 5
MoleculeChainsSequence LengthOrganismDetailsImage
ES1E [auth 4]214Lachancea kluyveriMutation(s): 0 
Gene Names: RPS1
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Entity ID: 6
MoleculeChainsSequence LengthOrganismDetailsImage
ES26F [auth 5]97Lachancea kluyveriMutation(s): 0 
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Entity ID: 7
MoleculeChainsSequence LengthOrganismDetailsImage
ES27G [auth 6]81Lachancea kluyveriMutation(s): 0 
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Entity ID: 8
MoleculeChainsSequence LengthOrganismDetailsImage
ES10H [auth 7]96Lachancea kluyveriMutation(s): 0 
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Entity ID: 9
MoleculeChainsSequence LengthOrganismDetailsImage
ES25I [auth 8]70Lachancea kluyveriMutation(s): 0 
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Entity ID: 10
MoleculeChainsSequence LengthOrganismDetailsImage
ES31J [auth 9]71Lachancea kluyveriMutation(s): 0 
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Entity ID: 12
MoleculeChainsSequence LengthOrganismDetailsImage
US2L [auth B]206Lachancea kluyveriMutation(s): 0 
Gene Names: RPS0
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Entity ID: 13
MoleculeChainsSequence LengthOrganismDetailsImage
US3M [auth C]223Lachancea kluyveriMutation(s): 0 
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Entity ID: 14
MoleculeChainsSequence LengthOrganismDetailsImage
US4N [auth D]185Lachancea kluyveriMutation(s): 0 
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Entity ID: 15
MoleculeChainsSequence LengthOrganismDetailsImage
US5O [auth E]217Lachancea kluyveriMutation(s): 0 
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Entity ID: 16
MoleculeChainsSequence LengthOrganismDetailsImage
EIF1P [auth F]83Lachancea kluyveriMutation(s): 0 
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Entity ID: 17
MoleculeChainsSequence LengthOrganismDetailsImage
US7Q [auth G]206Lachancea kluyveriMutation(s): 0 
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Entity ID: 18
MoleculeChainsSequence LengthOrganismDetailsImage
US8R [auth H]129Lachancea kluyveriMutation(s): 0 
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Entity ID: 19
MoleculeChainsSequence LengthOrganismDetailsImage
US9S [auth I]141Lachancea kluyveriMutation(s): 0 
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Entity ID: 20
MoleculeChainsSequence LengthOrganismDetailsImage
US10T [auth J]107Lachancea kluyveriMutation(s): 0 
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Entity ID: 21
MoleculeChainsSequence LengthOrganismDetailsImage
US11U [auth K]127Lachancea kluyveriMutation(s): 0 
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Entity ID: 22
MoleculeChainsSequence LengthOrganismDetailsImage
US12V [auth L]144Lachancea kluyveriMutation(s): 0 
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Entity ID: 23
MoleculeChainsSequence LengthOrganismDetailsImage
US13W [auth M]145Lachancea kluyveriMutation(s): 0 
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Entity ID: 24
MoleculeChainsSequence LengthOrganismDetailsImage
US14X [auth N]53Lachancea kluyveriMutation(s): 0 
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Entity ID: 25
MoleculeChainsSequence LengthOrganismDetailsImage
US15Y [auth O]150Lachancea kluyveriMutation(s): 0 
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Entity ID: 26
MoleculeChainsSequence LengthOrganismDetailsImage
ES24Z [auth P]134Lachancea kluyveriMutation(s): 0 
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Entity ID: 27
MoleculeChainsSequence LengthOrganismDetailsImage
US17AA [auth Q]155Lachancea kluyveriMutation(s): 0 
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Entity ID: 28
MoleculeChainsSequence LengthOrganismDetailsImage
RACK1BA [auth R]318Lachancea kluyveriMutation(s): 0 
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Entity ID: 29
MoleculeChainsSequence LengthOrganismDetailsImage
US19CA [auth S]124Lachancea kluyveriMutation(s): 0 
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Entity ID: 30
MoleculeChainsSequence LengthOrganismDetailsImage
ES19DA [auth T]143Lachancea kluyveriMutation(s): 0 
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Entity ID: 31
MoleculeChainsSequence LengthOrganismDetailsImage
ES12EA [auth U]124Lachancea kluyveriMutation(s): 0 
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Entity ID: 32
MoleculeChainsSequence LengthOrganismDetailsImage
ES17FA [auth V]120Lachancea kluyveriMutation(s): 0 
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Entity ID: 33
MoleculeChainsSequence LengthOrganismDetailsImage
ES4GA [auth W]260Lachancea kluyveriMutation(s): 0 
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Entity ID: 34
MoleculeChainsSequence LengthOrganismDetailsImage
ES30HA [auth X]60Lachancea kluyveriMutation(s): 0 
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Entity ID: 35
MoleculeChainsSequence LengthOrganismDetailsImage
ES6IA [auth Y]226Lachancea kluyveriMutation(s): 0 
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Entity ID: 36
MoleculeChainsSequence LengthOrganismDetailsImage
ES21JA [auth Z]87Lachancea kluyveriMutation(s): 0 
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Entity ID: 37
MoleculeChainsSequence LengthOrganismDetailsImage
EIF3AKA [auth a]964Lachancea kluyveriMutation(s): 0 
Gene Names: TIF32
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Entity ID: 38
MoleculeChainsSequence LengthOrganismDetailsImage
EIF3BLA [auth b]763Lachancea kluyveriMutation(s): 0 
Gene Names: PRT1
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Entity ID: 39
MoleculeChainsSequence LengthOrganismDetailsImage
EIF3CMA [auth c]812Lachancea kluyveriMutation(s): 0 
Gene Names: NIP1
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Entity ID: 11
MoleculeChainsLengthOrganismImage
18S RRNAK [auth A]1781Lachancea kluyveri
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Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 6.47 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2015-02-11
    Type: Initial release
  • Version 1.1: 2015-07-22
    Changes: Database references
  • Version 2.0: 2017-08-02
    Changes: Atomic model, Data collection, Derived calculations, Refinement description