5OPT

Structure of KSRP in context of Trypanosoma cruzi 40S


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 4.00 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

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This is version 1.1 of the entry. See complete history


Literature

The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.

Brito Querido, J.Mancera-Martinez, E.Vicens, Q.Bochler, A.Chicher, J.Simonetti, A.Hashem, Y.

(2017) Structure 25: 1785-1794.e3

  • DOI: 10.1016/j.str.2017.09.014
  • Primary Citation of Related Structures:  
    5OSG, 5OPT

  • PubMed Abstract: 
  • Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs ...

    Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized. Here, we present the near-atomic cryoelectron microscopy structure of a novel bona fide kinetoplastid-specific ribosomal (r-) protein (KSRP) bound to the ribosome. KSRP is an essential protein located at the solvent face of the 40S subunit, where it binds and stabilizes kinetoplastid-specific domains of rRNA, suggesting its role in ribosome integrity. KSRP also interacts with the r-protein eS6 at a region that is only conserved in kinetoplastids. The kinetoplastid-specific ribosomal environment of KSRP provides a promising target for the design of safer anti-kinetoplastidian drugs.


    Organizational Affiliation

    Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, 67000 Strasbourg, France. Electronic address: y.hashem@ibmc-cnrs.unistra.fr.



Macromolecules

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Entity ID: 1
MoleculeChainsSequence LengthOrganismDetailsImage
Activated protein kinase C receptor, putativeA [auth p]318Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511211.120Tc00.1047053511211.130
Find proteins for Q4DTN2 (Trypanosoma cruzi (strain CL Brener))
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Entity ID: 2
MoleculeChainsSequence LengthOrganismDetailsImage
Ribosomal protein S29, putativeB [auth q]57Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506025.14Tc00.1047053511209.54Tc00.1047053511805.15
Find proteins for Q4CXR4 (Trypanosoma cruzi (strain CL Brener))
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Entity ID: 3
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S16, putativeC [auth r]149Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053503899.20Tc00.1047053503899.30
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Entity ID: 4
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S15, putativeD [auth t]152Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511809.130Tc00.1047053511809.99Tc00.1047053511811.10
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Entity ID: 5
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S18, putativeE [auth u]153Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506679.100Tc00.1047053506679.94
Find proteins for Q4E093 (Trypanosoma cruzi (strain CL Brener))
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Entity ID: 6
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S4F [auth L]273Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053509683.117Tc00.1047053511051.39Tc00.1047053511051.50
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Entity ID: 7
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S23, putativeG [auth M]143Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053504181.20Tc00.1047053504181.30Tc00.1047053509695.180Tc00.1047053509695.184
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Entity ID: 8
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S5, putativeH [auth O]190Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506297.150Tc00.1047053506297.160
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Entity ID: 9
MoleculeChainsSequence LengthOrganismDetailsImage
Ribosomal protein S7, putativeI [auth Q]211Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506593.30
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Entity ID: 10
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S13, putativeJ [auth R]151Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511291.90
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Entity ID: 11
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S27, putativeK [auth S]86Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506963.14Tc00.1047053511233.14Tc00.1047053511277.175
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Entity ID: 12
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S26L [auth T]112Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053503801.20Tc00.1047053508817.60
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Entity ID: 13
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S33, putativeM [auth U]112Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511237.60
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Entity ID: 14
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S14, putativeN [auth V]144Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053409117.20Tc00.1047053506945.230Tc00.1047053508823.50
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Entity ID: 15
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40S ribosomal protein S3a-2O [auth W]261Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511001.9Tc00.1047053511001.18
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Entity ID: 16
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S11, putativeP [auth X]173Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053508319.70Tc00.1047053508405.40
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Entity ID: 17
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S24Q [auth Y]137Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053507681.150Tc00.1047053507681.160
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Entity ID: 18
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S8R [auth Z]221Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511903.110
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Entity ID: 19
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S9, putativeS [auth b]190Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053504163.30Tc00.1047053506401.120
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Entity ID: 20
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein SAT [auth f]245Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053510425.19
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Entity ID: 21
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S2, putativeU [auth d]263Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053503833.40Tc00.1047053506213.60Tc00.1047053511287.120Tc00.1047053511287.40
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Entity ID: 22
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S15a, putativeV [auth e]130Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506297.330
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Entity ID: 23
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S21, putativeW [auth g]236Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053510101.420
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Entity ID: 24
MoleculeChainsSequence LengthOrganismDetailsImage
Ribosomal protein S25, putativeX [auth a]110Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053504105.94Tc00.1047053509233.190
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Entity ID: 25
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S12Y [auth i]141Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053503395.50Tc00.1047053506181.59
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Entity ID: 26
MoleculeChainsSequence LengthOrganismDetailsImage
Ubiquitin/ribosomal protein S27a, putativeZ [auth j]150Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053510409.39
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Entity ID: 27
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S6AA [auth P]250Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053510769.49
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Entity ID: 28
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S17, putativeBA [auth k]196Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053508827.70
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Entity ID: 29
MoleculeChainsSequence LengthOrganismDetailsImage
Ribosomal protein S20, putativeCA [auth l]117Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053508475.10Tc00.1047053508823.120Tc00.1047053508823.140
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Entity ID: 30
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S3, putativeDA [auth m]214Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053504021.109Tc00.1047053507677.39Tc00.1047053509353.30
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Entity ID: 31
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S10, putativeEA [auth n]161Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053506679.140Tc00.1047053506679.150
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Entity ID: 32
MoleculeChainsSequence LengthOrganismDetailsImage
Ribosomal protein S19, putativeFA [auth o]167Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053510879.20
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Entity ID: 33
MoleculeChainsSequence LengthOrganismDetailsImage
40S ribosomal protein S30GA [auth c]66Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053507019.83Tc00.1047053507019.86
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Entity ID: 34
MoleculeChainsSequence LengthOrganismDetailsImage
RNA-binding protein, putativeHA [auth h]257Trypanosoma cruzi strain CL BrenerMutation(s): 0 
Gene Names: Tc00.1047053511727.270
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Entity ID: 35
MoleculeChainsLengthOrganismImage
18S rRNAIA [auth E]2319Trypanosoma cruzi strain CL Brener
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 4.00 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
The French National Research AgencyFranceANR-14-ACHN-0024
LABEXFranceANR-10-LABX-0036_NETRNA

Revision History  (Full details and data files)

  • Version 1.0: 2017-11-15
    Type: Initial release
  • Version 1.1: 2017-12-13
    Changes: Database references