Download MendeleyCa 2+ -mediated higher-order assembly of b 0,+ AT-rBAT is a key step for system b 0,+ biogenesis and cystinuria
Lee, Y., Wiriyasermkul, P., Moriyama, S., Mills, D.J., Kuhlbrandt, W., Nagamori, S.(2021) Biorxiv
7NF8
Ovine (b0,+AT-rBAT)2 hetero-tetramer, asymmetric unit, rigid-body fitted
- PDB DOI: 10.2210/pdb7NF8/pdb
- EM Map EMD-12298:EMDBEMDataResource
- Classification: TRANSLOCASE
- Organism(s): Ovis aries
- Expression System: Homo sapiens
- Mutation(s): No
- Membrane Protein: Yes PDBTMMemProtMD
- Deposited: 2021-02-05 Released: 2022-01-19
- Funding Organization(s): Max Planck Society, Human Frontier Science Program (HFSP)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 2.83 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| B(0,+)-type amino acid transporter 1 | A [auth B] | 515 | Ovis aries | Mutation(s): 0 Gene Names: SLC7A9, JEQ12_005167 Membrane Entity: Yes |  |
UniProt | |||||
Find proteins for A0A6P3EL78 (Ovis aries) Explore A0A6P3EL78 Go to UniProtKB: A0A6P3EL78 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A6P3EL78 | ||||
Protein Feature ViewExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| neutral and basic amino acid transport protein rBAT | B [auth A] | 686 | Ovis aries | Mutation(s): 0 Gene Names: SLC3A1, JEQ12_014007 Membrane Entity: Yes |  |
UniProt | |||||
Find proteins for A0A6P7DVK7 (Ovis aries) Explore A0A6P7DVK7 Go to UniProtKB: A0A6P7DVK7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A6P7DVK7 | ||||
Protein Feature ViewExpand | |||||
| |||||
Oligosaccharides
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Chain Length | 2D Diagram | Glycosylation | 3D Interactions |
| 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | C, D | 2 |  | N-Glycosylation | Oligosaccharides Interaction |
Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| LBN Query on LBN | G [auth A] | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine C42 H82 N O8 P WTJKGGKOPKCXLL-VYOBOKEXSA-N |  | Ligand Interaction | |
| CLR Query on CLR | E [auth B], F [auth B] | CHOLESTEROL C27 H46 O HVYWMOMLDIMFJA-DPAQBDIFSA-N |  | Ligand Interaction | |
| NAG Query on NAG | I [auth A], J [auth A], K [auth A], L [auth A] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | Ligand Interaction | |
| CA (Subject of Investigation/LOI) Query on CA | H [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | Ligand Interaction | |
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 2.83 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Entry History & Funding Information
Deposition Data
- Deposited Date: 2021-02-05
- Released Date: 2022-01-19 Deposition Author(s): Lee, Y., Kuehlbrandt, W.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Max Planck Society | Germany | -- |
| Human Frontier Science Program (HFSP) | -- |
Revision History (Full details and data files)
- Version 1.0: 2022-01-19
Type: Initial release
