Download MendeleyMycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
Delbeau, M., Campbell, E.A., Darst, S.A.To be published.
8E74
Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
- PDB DOI: https://doi.org/10.2210/pdb8E74/pdb
- EM Map EMD-27935: EMDB EMDataResource
- Classification: TRANSCRIPTION/DNA/RNA
- Organism(s): Mycobacterium tuberculosis, synthetic construct
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2022-08-23 Released: 2023-03-22
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 2.94 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Transcription termination/antitermination protein NusG | A [auth Z] | 238 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: nusG, ERS007663_02289, ERS007665_03629, ERS007670_00511, ERS007679_03447, ERS007681_03562, ERS007703_00852, ERS007741_01181, ERS024276_01409, ERS027646_02899... |  |
UniProt | |||||
Find proteins for A0A045HU92 (Mycobacterium tuberculosis) Explore A0A045HU92 Go to UniProtKB: A0A045HU92 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A045HU92 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit alpha | B [auth A], C [auth B] | 347 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoA EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A5U8D3 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)) Explore A5U8D3 Go to UniProtKB: A5U8D3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A5U8D3 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta | D [auth C] | 1,172 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoB, MRA_0676 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A5U052 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)) Explore A5U052 Go to UniProtKB: A5U052 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A5U052 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta' | E [auth D] | 1,318 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoC, E5M52_05480, ERS007665_00591, ERS013471_00574, ERS023446_00410, ERS075361_00813, ERS094182_01340, F6W99_03342, E5M05_06640, E5M23_08570... EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A0A045J9E2 (Mycobacterium tuberculosis) Explore A0A045J9E2 Go to UniProtKB: A0A045J9E2 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A045J9E2 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 5 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit omega | F [auth E] | 110 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoZ, ERS007703_04032, ERS007720_04749, ERS094182_01030 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A0A0T9N9K3 (Mycobacterium tuberculosis) Explore A0A0T9N9K3 Go to UniProtKB: A0A0T9N9K3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A0T9N9K3 | ||||
Protein Feature ViewExpand | |||||
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Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 6 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| DNA (54-MER) | G [auth O] | 54 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 7 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| DNA (54-MER) | H [auth P] | 54 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 8 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| RNA (42-MER) | I [auth R] | 42 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZN Query on ZN | J [auth D], K [auth D] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | Ligand Interaction | |
| MG Query on MG | L [auth D] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | Ligand Interaction | |
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 2.94 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Entry History & Funding Information
Deposition Data
- Released Date: 2023-03-22 Deposition Author(s): Delbeau, M., Darst, S.A., Campbell, E.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | -- |
Revision History (Full details and data files)
- Version 1.0: 2023-03-22
Type: Initial release
