8E79

Mycobacterium tuberculosis RNAP paused elongation complex with Escherichia coli NusG transcription factor


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.71 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.0 of the entry. See complete history


Literature

Mycobacterium tuberculosis RNAP paused elongation complex with Escherichia coli NusG transcription factor

Delbeau, M.Campbell, E.A.Darst, S.A.

To be published.

Macromolecules

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Entity ID: 1
MoleculeChainsSequence LengthOrganismDetailsImage
DNA-directed RNA polymerase subunit alphaA, B347Mycobacterium tuberculosisMutation(s): 0 
Gene Names: rpoA
EC: 2.7.7.6
UniProt
Find proteins for A5U8D3 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra))
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UniProt GroupA5U8D3
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Entity ID: 2
MoleculeChainsSequence LengthOrganismDetailsImage
DNA-directed RNA polymerase subunit betaC1,172Mycobacterium tuberculosisMutation(s): 0 
Gene Names: rpoBMRA_0676
EC: 2.7.7.6
UniProt
Find proteins for A5U052 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra))
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UniProt GroupA5U052
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Entity ID: 3
MoleculeChainsSequence LengthOrganismDetailsImage
DNA-directed RNA polymerase subunit beta'D1,318Mycobacterium tuberculosisMutation(s): 0 
Gene Names: 
EC: 2.7.7.6
UniProt
Find proteins for A0A045J9E2 (Mycobacterium tuberculosis)
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UniProt GroupA0A045J9E2
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Entity ID: 4
MoleculeChainsSequence LengthOrganismDetailsImage
DNA-directed RNA polymerase subunit omegaE110Mycobacterium tuberculosisMutation(s): 0 
Gene Names: rpoZERS007703_04032ERS007720_04749ERS094182_01030
EC: 2.7.7.6
UniProt
Find proteins for A0A0T9N9K3 (Mycobacterium tuberculosis)
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UniProt GroupA0A0T9N9K3
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Entity ID: 5
MoleculeChainsSequence LengthOrganismDetailsImage
Transcription termination/antitermination protein NusGF [auth N]181Escherichia coliMutation(s): 0 
Gene Names: nusG
UniProt
Find proteins for U9XYQ6 (Escherichia coli 113303)
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UniProt GroupU9XYQ6
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Entity ID: 6
MoleculeChainsLengthOrganismImage
DNA (54-MER)G [auth O]54synthetic construct
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Entity ID: 7
MoleculeChainsLengthOrganismImage
DNA (54-MER)H [auth P]54synthetic construct
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Entity ID: 8
MoleculeChainsLengthOrganismImage
RNA (42-MER)I [auth R]42synthetic construct
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Small Molecules
Ligands 2 Unique
IDChainsName / Formula / InChI Key2D Diagram3D Interactions
ZN
Query on ZN

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J [auth D],
K [auth D]
ZINC ION
Zn
PTFCDOFLOPIGGS-UHFFFAOYSA-N
 Ligand Interaction
MG
Query on MG

Download Ideal Coordinates CCD File 
L [auth D]MAGNESIUM ION
Mg
JLVVSXFLKOJNIY-UHFFFAOYSA-N
 Ligand Interaction
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 3.71 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

Structure Validation

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Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)United States--

Revision History  (Full details and data files)

  • Version 1.0: 2023-03-22
    Type: Initial release