Download MendeleyMycobacterium tuberculosis RNAP paused elongation complex with Escherichia coli NusG transcription factor
Delbeau, M., Campbell, E.A., Darst, S.A.To be published.
8E79
Mycobacterium tuberculosis RNAP paused elongation complex with Escherichia coli NusG transcription factor
- PDB DOI: https://doi.org/10.2210/pdb8E79/pdb
- EM Map EMD-27938: EMDB EMDataResource
- Classification: TRANSCRIPTION/DNA/RNA
- Organism(s): Mycobacterium tuberculosis, Escherichia coli, synthetic construct
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2022-08-23 Released: 2023-03-22
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 3.71 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit alpha | A, B | 347 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoA EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A5U8D3 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)) Explore A5U8D3 Go to UniProtKB: A5U8D3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A5U8D3 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta | C | 1,172 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoB, MRA_0676 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A5U052 (Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)) Explore A5U052 Go to UniProtKB: A5U052 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A5U052 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta' | D | 1,318 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoC, E5M52_05480, ERS007665_00591, ERS013471_00574, ERS023446_00410, ERS075361_00813, ERS094182_01340, F6W99_03342, E5M05_06640, E5M23_08570... EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A0A045J9E2 (Mycobacterium tuberculosis) Explore A0A045J9E2 Go to UniProtKB: A0A045J9E2 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A045J9E2 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit omega | E | 110 | Mycobacterium tuberculosis | Mutation(s): 0 Gene Names: rpoZ, ERS007703_04032, ERS007720_04749, ERS094182_01030 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for A0A0T9N9K3 (Mycobacterium tuberculosis) Explore A0A0T9N9K3 Go to UniProtKB: A0A0T9N9K3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A0T9N9K3 | ||||
Protein Feature ViewExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 5 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Transcription termination/antitermination protein NusG | F [auth N] | 181 | Escherichia coli | Mutation(s): 0 Gene Names: nusG |  |
UniProt | |||||
Find proteins for U9XYQ6 (Escherichia coli 113303) Explore U9XYQ6 Go to UniProtKB: U9XYQ6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | U9XYQ6 | ||||
Protein Feature ViewExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 6 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| DNA (54-MER) | G [auth O] | 54 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 7 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| DNA (54-MER) | H [auth P] | 54 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 8 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| RNA (42-MER) | I [auth R] | 42 | synthetic construct |  | |
Protein Feature ViewExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZN Query on ZN | J [auth D], K [auth D] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | Ligand Interaction | |
| MG Query on MG | L [auth D] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | Ligand Interaction | |
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 3.71 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Entry History & Funding Information
Deposition Data
- Released Date: 2023-03-22 Deposition Author(s): Delbeau, M., Darst, S.A., Campbell, E.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | -- |
Revision History (Full details and data files)
- Version 1.0: 2023-03-22
Type: Initial release
